Ex Vivo Activity of Cardiac Glycosides in Acute Leukaemia
نویسندگان
چکیده
BACKGROUND Despite years of interest in the anti-cancerous effects of cardiac glycosides (CGs), and numerous studies in vitro and in animals, it has not yet been possible to utilize this potential clinically. Reports have demonstrated promising in vitro effects on different targets as well as a possible therapeutic index/selectivity in vitro and in experimental animals. Recently, however, general inhibition of protein synthesis was suggested as the main mechanism of the anti-cancerous effects of CGs. In addition, evidence of species differences of a magnitude sufficient to explain the results of many studies called for reconsideration of earlier results. PRINCIPAL FINDINGS In this report we identified primary B-precursor and T-ALL cells as being particularly susceptible to the cytotoxic effects of CGs. Digitoxin appeared most potent and IC(50) values for several patient samples were at concentrations that may be achieved in the clinic. Significant protein synthesis inhibition at concentrations corresponding to IC(50) was demonstrated in colorectal tumour cell lines moderately resistant to the cytotoxic effects of digoxin and digitoxin, but not in highly sensitive leukaemia cell lines. CONCLUSION It is suggested that further investigation regarding CGs may be focused on diagnoses like T- and B-precursor ALL.
منابع مشابه
Linarin Inhibits the Acetylcholinesterase Activity In-vitro and Ex-vivo
Linarin is a flavone glycoside in the plants Flos chrysanthemi indici, Buddleja officinalis, Cirsium setosum, Mentha arvensis and Buddleja davidii, and has been reported to possess analgesic, antipyretic, anti-inflammatory and neuroprotective activities. In this paper, linarin was investigated for its AChE inhibitory potential both in vitro and ex vivo. Ellman’s colorimetric method was used for...
متن کاملLinarin Inhibits the Acetylcholinesterase Activity In-vitro and Ex-vivo
Linarin is a flavone glycoside in the plants Flos chrysanthemi indici, Buddleja officinalis, Cirsium setosum, Mentha arvensis and Buddleja davidii, and has been reported to possess analgesic, antipyretic, anti-inflammatory and neuroprotective activities. In this paper, linarin was investigated for its AChE inhibitory potential both in vitro and ex vivo. Ellman’s colorimetric method was used for...
متن کاملEffect of endurance training with cinnamon bark extract on antioxidant activity levels and cardiac index in streptozotocin- (STZ) induced DM male rats
Background: Diabetes may be associated with an imbalance between the protective effects of antioxidants and increased production of free radicals. Oxidative stress also appears to contribute to the pathogenesis of diabetic complications. In this study the effect of endurance exercise with cinnamon bark extract on antioxidant activity levels and cardiac index in streptozotocin- (STZ) induced DM ...
متن کاملشناسایی گلیکوزیدهای قلبی گونه دیژیتال ایران به روش HPLC
Background and Purpose: Digitalis species and their cardiac glycosides are used in congestive heart failure (ÇHF) disease. Digitalis nervosa are grown only in the North regions of Ïran. Çardiac glycosides of other species are identified by HPLÇ procedure. Ïn this research, cardiac glycosides of Digitalis nervosa were identified. Materials and Methods: Ïn this research, methanol as solvent ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 6 شماره
صفحات -
تاریخ انتشار 2011